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KMID : 1005220110260010019
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Journal of Embryo Transfer
2011 Volume.26 No. 1 p.19 ~ p.25
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Effect of Early Pregnant Heifer as Donor on the Ovum Pick-Up Derived Oocyte Aspiration and Embryo Production
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Jin Jong-In
Kwon Tae-Hyeon
Choi Byeong-Hyun
Kim Sung-Soo
Jo Hyun-Tae
Bang Jae-Il
Kim Sam-Churl
Cho Kyu-Woan
Lee Jung-Gyu
Kong Il-Keun
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Abstract
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This study was carried out to evaluate the effect of early pregnant cow as donor for Ovum Pick-Up (OPU) derived oocyte aspiration and embryo production in Holstein heifers. Four non-pregnant and 2 pregnant Holstein heifers were used as donor and then carried out total 17 OPU session for 10 weeks (2 times per week). Recovered cumulus-oocyte-complexes (COCs) were classified into 4 grade by oocyte cytoplasm and cumulus cells and matured in vitro in TCM-199 supplemented with 10% FBS, 10 mg/ml FSH and 1 mg/ml estradiol in 5% CO₂and over 99% humidity for 24 h. After 24 h co-incubation with post-thaw sperm, the presumed zygotes were cultured in CR1aa medium with 4 mg/ml BSA for 3 days and then changed CR1aa medium with 10% of FBS for another 3~4 days. The Mean number of aspirated follicles and collected oocytes in the early stage pregnant and non-pregnant heifers were 13.0±4.3 and 10.6±3.9, 5.4±3.4 and 7.7±3.6 per session, respectively. Rate of collected oocyte from aspirated follicles were 59.2% and 50.5%, respectively. The average number of good quality oocytes (Grade I and II) in the early stage pregnant and non-pregnant heifers was 3.7±2.7 and 4.9±2.6 (Mean±SD). Cleavage and blastocyst developmental rates in Grade I and II were 22.2% and 25.5%, and then 1.7±0.9 and 1.4±1.1 blastocyst per session, respectively. In conclusion, OPU technology can be used in early stage pregnant and non-pregnant heifers without any problem and so applied OPU derived embryo production to maximize the ability of genetically valuable females.
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KEYWORD
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OPU, Holstein, heifer, oocyte, pregnant donor
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